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Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study

Chen, J., Lan, P., Tarr, A., Yan, Y.M., Francki, M., Appels, R. and Ma, W.ORCID: 0000-0002-1264-866X (2007) Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study. Rapid Communications in Mass Spectrometry, 21 (17). pp. 2913-2917.

Link to Published Version: http://dx.doi.org/10.1002/rcm.3151
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Abstract

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrumentation has been used to analyze wheat seed gliadins as an alternative to other established methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), capillary electrophoresis (CE), high-performance liquid chromatography (HPLC), etc. The MALDI-TOF approach has shown to have many advantages such as high resolution, cost effectiveness and high throughput. MALDI-TOF-based gliadin profiles have been used for fast wheat cultivar identification. However, the genetic information represented by individual gliadin peaks has not been utilized. In this study a wheat doubled haploid population with a genetic linkage map of good coverage was used to assay individual gliadin peaks from MALDI-TOF profiles as molecular markers. Eight segregating peaks in the population were scored as polymorphic across the population. The 1 to 1 segregating ratios validated the scoring of the peaks and all peaks were mapped to the expected chromosomes or linkage groups on the available linkage map: 1 peak on chromosome 1A, 1 on 6A, 4 on 6B and 2 on 6D.

Item Type: Journal Article
Murdoch Affiliation: Centre for Comparative Genomics
Publisher: John Wiley and Sons Ltd
Copyright: © 2007 John Wiley & Sons, Ltd.
URI: http://researchrepository.murdoch.edu.au/id/eprint/9127
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