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Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo

Setiyaningsih, Surachmi, Desport, M., Stewart, M.E., Hartaningsih, N. and Wilcox, G.E. (2008) Sequence analysis of mRNA transcripts encoding Jembrana disease virus Tat-1 in vivo. Virus Research, 132 (1-2). pp. 220-225.

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Jembrana disease virus (JDV) is a lentivirus which replicates to very high titres in vivo and its Tat-1 protein has been shown to be a potent transactivator in vitro. Analysis of tat mRNA transcripts produced early in infection studies identified four predominant species which were generated by multiple splicing events. The use of a splice donor downstream of tat-1 was common indicating that a Tat-1 protein of 97 amino acids is expressed during the acute phase of Jembrana disease. The presence of an in-frame stop codon between tat-1 and tat-2 was identified in transcripts from three different strains of JDV confirming that expression of a single exon Tat-1 correlates with high viral titres in vivo. Sequence conservation in the regions of tat-1 that are critical for RNA binding and transcription activation in three different virus strains was high and the tat-2 sequences were completely conserved.

Item Type: Journal Article
Murdoch Affiliation(s): School of Veterinary and Biomedical Sciences
Publisher: Elsevier BV
Copyright: 2007 Elsevier BV
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