Isolation and in vitro translation of messenger RNA encoding allergens of the cat flea, Ctenocephalides felis
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Greene, W.K., Penhale, W.J. and Thompson, R.C.A. (1993) Isolation and in vitro translation of messenger RNA encoding allergens of the cat flea, Ctenocephalides felis. Veterinary Immunology and Immunopathology, 37 (1). pp. 15-23.
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Abstract
Polyadenylated [poly(A)+] mRNA was isolated from the cat flea, Ctenocephalides felis by oligothymidylic acid-cellulose spin column chromatography and translated in vitro using a cell-free rabbit reticulocyte system. The relative incorporation of 35S-methionine into trichloroacetic acid-precipitable translation products obtained using poly (A+) mRNA was 48.5-fold over control translations performed without added mRNA. SDS-PAGE analysis of the translation products in combination with autoradiography showed that many proteins with apparent molecular weights in the range 14-90 K were synthesized. Immunoprecipitation studies performed using flea-allergic dog sera showed that several of the synthesized proteins corresponded with flea allergens. The allergenicity of the lysates was also confirmed by skin testing.
| Item Type: | Journal Article |
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| Murdoch Affiliation(s): | School of Veterinary Studies |
| Publisher: | Elsevier BV |
| Copyright: | © Elsevier B.V. |
| URI: | http://researchrepository.murdoch.edu.au/id/eprint/7520 |
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