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Development of wheat–Lophopyrum elongatum recombinant lines for enhanced sodium 'exclusion' during salinity stress

Mullan, D.J., Mirzaghaderi, G., Walker, E., Colmer, T.D. and Francki, M.G. (2009) Development of wheat–Lophopyrum elongatum recombinant lines for enhanced sodium 'exclusion' during salinity stress. Theoretical and Applied Genetics, 119 (7). pp. 1313-1323.

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Lophopyrum elongatum (tall wheatgrass), a wild relative of wheat, can be used as a source of novel genes for improving salt tolerance of bread wheat. Sodium 'exclusion' is a major physiological mechanism for salt tol- erance in a wheat-tall wheatgrass amphiploid, and a large proportion (~50%) for reduced Na+ accumulation in the Xag leaf, as compared to wheat, was earlier shown to be contributed by genetic eVects from substitution of chromosome 3E from tall wheatgrass for wheat chromosomes 3A and 3D. Homoeologous recombination between 3E and wheat chromosomes 3A and 3D was induced using the ph1b mutant, and putative recombinants were identiWed as having SSR markers speciWc for tall wheatgrass loci. As many as 14 recombinants with smaller segments of tall wheatgrass chromatin were identiWed and low-resolution breakpoint analysis was achieved using wheat SSR loci. Seven recombinants were identiWed to have leaf Na+ concentrations similar to those in 3E(3A) or 3E(3D) substitution lines, when grown in 200 mM NaCl in nutrient solution. Phenotypic analysis identiWed recombinants with introgressions at the distal end on the long arm of homoeologous group 3 chromosomes being responsible for Na+ 'exclusion'. A total of 55 wheat SSR markers mapped to the long arm of homoeologous group 3 markers by genetic and deletion bin mapping were used for high resolution of wheat-tall wheatgrass chromosomal breakpoints in selected recombinants. Molecular marker analysis and genomic in situ hybridisation conWrmed the 524-568 recombinant line as containing the smallest introgression of tall wheatgrass chromatin on the distal end of the long arm of wheat chromosome 3A and identiWed this line as suitable for developing wheat germplasm with Na+ 'exclusion'.

Item Type: Journal Article
Murdoch Affiliation(s): Western Australian State Agricultural Biotechnology Centre
Publisher: Springer Verlag
Copyright: © Springer-Verlag 2009
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