Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples

Wylie, S.J.ORCID: 0000-0002-5639-7460, Luo, H., Li, H. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 (2012) Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples. Archives of Virology, 157 (2). pp. 271-284.

Link to Published Version:
*Subscription may be required


RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus, Nepovirus,Allexivirus, and Carlavirus. Four were unknown and are proposed as members of the genera Potyvirus, Sadwavirus, and Trichovirus. Virus sequences were subsequently matched to original host plants using RT-PCR assays.

Item Type: Journal Article
Murdoch Affiliation(s): Western Australian State Agricultural Biotechnology Centre
School of Biological Sciences and Biotechnology
Publisher: Springer Verlag
Copyright: © 2011 Springer-Verlag
Item Control Page Item Control Page