Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Ancient DNA extraction from soils and sediments

Haile, J. (2012) Ancient DNA extraction from soils and sediments. Methods in molecular biology, 840 . pp. 57-63.

Link to Published Version:
*Subscription may be required


DNA contained in soils and sediments can provide novel insights into past environments and ecosystems. In this chapter, I describe an efficient and effective technique to extract total DNA from sediments in a manner that minimizes the coextraction of PCR-inhibitory compounds. I describe two different approaches: one that is suitable for large (up to 10 g wet weight) amounts of substrate, and a second that is more appropriate for small (up to 0.5 g) amounts of substrate. Finally, I discuss some of the obstacles that may be encountered in the process of extracting DNA from soils and sediments and suggest approaches to circumvent some common problems.

Item Type: Journal Article
Murdoch Affiliation(s): School of Biological Sciences and Biotechnology
Publisher: Humana Press, Inc.
Copyright: © 2012 Springer Science+Business Media, LLC
Item Control Page Item Control Page