Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Small RNA analyses of a ceratobasidium isolate infected with three endornaviruses

Cao, C.T.H., Derbyshire, M.C., Regmi, R., Li, H., Jones, M.G.K.ORCID: 0000-0001-5002-0227 and Wylie, S.J.ORCID: 0000-0002-5639-7460 (2022) Small RNA analyses of a ceratobasidium isolate infected with three endornaviruses. Viruses, 14 (10). Article 2276.

PDF - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
Free to read:
*No subscription required


Isolates of three endornavirus species were identified co-infecting an unidentified species of Ceratobasidium, itself identified as a symbiont from within the roots of a wild plant of the terrestrial orchid Pterostylis vittata in Western Australia. Isogenic lines of the fungal isolate lacking all three mycoviruses were derived from the virus-infected isolate. To observe how presence of endornaviruses influenced gene expression in the fungal host, we sequenced fungus-derived small RNA species from the virus-infected and virus-free isogenic lines and compared them. The presence of mycoviruses influenced expression of small RNAs. Of the 3272 fungus-derived small RNA species identified, the expression of 9.1% (300 of 3272) of them were up-regulated, and 0.6% (18 of 3272) were down-regulated in the presence of the viruses. Fourteen novel micro-RNA-like RNAs (Cer-milRNAs) were predicted. Gene target prediction of the differentially expressed Cer-milRNAs was quite ambiguous; however, fungal genes involved in transcriptional regulation, catalysis, molecular binding, and metabolic activities such as gene expression, DNA metabolic processes and regulation activities were differentially expressed in the presence of the mycoviruses.

Item Type: Journal Article
Murdoch Affiliation(s): State Agricultural Biotechnology Centre
Publisher: MDPI
Copyright: © 2022 by the authors
Item Control Page Item Control Page


Downloads per month over past year