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Physical measurements of the liver glucocorticoid receptor

Litwack, G., Cake, M.H.ORCID: 0000-0002-5899-7291, Filler, R. and Taylor, K. (1978) Physical measurements of the liver glucocorticoid receptor. Biochemical Journal, 169 (2). pp. 445-448.

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Physical measurements were made on the cytosolic form of the liver [3H]dexamethasone receptor. These include a Stokes radius of 3.5 nm, determined by gel filtration, and sedimentation coefficients of 5.1 and 7-8S, by sucrose-density-gradient centrifugation. From these measurements, the following physical properties were calculated: apparent mol. wt. 78000 (the 5.1 S form); D app. 6.1 × 10(-7) cm2-S-1; f/fo 1.25; axial ratio 4.7; these indicate a globular protein. Measurements of sedimentation coefficient of cytosol steroid-receptor complexes previously subjected to various activating conditions gave different values and lead to the conclusion that the mechanism of activation in vitro enabling the steroid-receptor complex to bind to DNA is more complex than simple disaggregation to a uniform size.

Item Type: Journal Article
Publisher: Portland Press, Ltd.
Copyright: © 1978 Portland Press
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