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GS-2: A novel broad-spectrum agent for environmental microbial control

Mayfosh, A.J., Day, Z.I., Unsworth, N.B., Liu, C-Q, Gupta, R., Haynes, S., Abraham, R., Abraham, S., Shaw, Z.L., Walia, S., Elbourne, A., Hulett, M.D. and Rau, T.F. (2022) GS-2: A novel broad-spectrum agent for environmental microbial control. Biomolecules, 12 (9). Article 1293.

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The environmental control of microbial pathogens currently relies on compounds that do not exert long-lasting activity on surfaces, are impaired by soil, and contribute to the growing problem of antimicrobial resistance. This study presents the scientific development and characterization of GS-2, a novel, water-soluble ammonium carboxylate salt of capric acid and L-arginine that demonstrates activity against a range of bacteria (particularly Gram-negative bacteria), fungi, and viruses. In real-world surface testing, GS-2 was more effective than a benzalkonium chloride disinfectant at reducing the bacterial load on common touch-point surfaces in a high-traffic building (average 1.6 vs. 32.6 CFUs recovered from surfaces 90 min after application, respectively). Toxicology testing in rats confirmed GS-2 ingredients were rapidly cleared and posed no toxicities to humans or animals. To enhance the time-kill against Gram-positive bacteria, GS-2 was compounded at a specific ratio with a naturally occurring monoterpenoid, thymol, to produce a water-based antimicrobial solution. This GS-2 with thymol formulation could generate a bactericidal effect after five minutes of exposure and a viricidal effect after 10 min of exposure. Further testing of the GS-2 and thymol combination on glass slides demonstrated that the compound retained bactericidal activity for up to 60 days. Based on these results, GS-2 and GS-2 with thymol represent a novel antimicrobial solution that may have significant utility in the long-term reduction of environmental microbial pathogens in a variety of settings.

Item Type: Journal Article
Murdoch Affiliation(s): Harry Butler Institute
Antimicrobial Resistance and Infectious Disease Laboratory
Publisher: MDPI
Copyright: © 2022 by the authors
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