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Combined proteomic and metabolomic analysis of the molecular mechanism underlying the response to salt stress during seed germination in barley

Chen, Y., Wang, J., Yao, L., Li, B., Ma, X., Si, E., Yang, K., Li, C., Shang, X., Meng, Y. and Wang, H. (2022) Combined proteomic and metabolomic analysis of the molecular mechanism underlying the response to salt stress during seed germination in barley. International Journal of Molecular Sciences, 23 (18). Article 10515.

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Salt stress is a major abiotic stress factor affecting crop production, and understanding of the response mechanisms of seed germination to salt stress can help to improve crop tolerance and yield. The differences in regulatory pathways during germination in different salt-tolerant barley seeds are not clear. Therefore, this study investigated the responses of different salt-tolerant barley seeds during germination to salt stress at the proteomic and metabolic levels. To do so, the proteomics and metabolomics of two barley seeds with different salt tolerances were comprehensively examined. Through comparative proteomic analysis, 778 differentially expressed proteins were identified, of which 335 were upregulated and 443 were downregulated. These proteins, were mainly involved in signal transduction, propanoate metabolism, phenylpropanoid biosynthesis, plant hormones and cell wall stress. In addition, a total of 187 salt-regulated metabolites were identified in this research, which were mainly related to ABC transporters, amino acid metabolism, carbohydrate metabolism and lipid metabolism; 72 were increased and 112 were decreased. Compared with salt-sensitive materials, salt-tolerant materials responded more positively to salt stress at the protein and metabolic levels. Taken together, these results suggest that salt-tolerant germplasm may enhance resilience by repairing intracellular structures, promoting lipid metabolism and increasing osmotic metabolites. These data not only provide new ideas for how seeds respond to salt stress but also provide new directions for studying the molecular mechanisms and the metabolic homeostasis of seeds in the early stages of germination under abiotic stresses.

Item Type: Journal Article
Murdoch Affiliation(s): Western Crop Genetics Alliance
College of Science, Health, Engineering and Education
Publisher: MDPI
Copyright: © 2022 by the authors
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