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Development of a total flavonoids extract of Artemisia Rupestris L. via nanotechnology and its antiviral effect In Vitro

Hamulati, H., Chen, S., Gu, Z., Sun, Y. and Wang, T. (2020) Development of a total flavonoids extract of Artemisia Rupestris L. via nanotechnology and its antiviral effect In Vitro. Infectious Diseases Research, 1 (2).

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Free to read: https://doi.org/10.53388/IDR20200802006
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Abstract

Objective: We aimed to develop a novel Artemisia rupestris L. flavonoid nano-encapsulation (AFN) preparation and evaulate its anti-hepatitis B virus (HBV) activityin vitro. Methods: First, the AFN was prepared using polylactic-co-glycolic acid (PLGA). Then, after verification of the AFN, in vitro anti-virus assays were conducted by: (1) assessing the inhibitory effect of AFN on the secretion of hepatitis B surface antigens (HBsAg), hepatitis Be-antigens (HBeAg), and the replication of HBV DNAin HepG2.2.15 cells; (2) analyzing the influence of AFN on the activation rate of NF-κB positive cells; and (3) evaluating the effect of AFN on the function of glutathione peroxidase (GSH-PX) enzymes located onthe HepG2.2.15 cell membrane. Results: Compared to the original total flavonoids extract of Artemisia rupestris L. (withoutnano-encapsulation), AFN preparation under the maximum non-toxic concentration effectively inhibited the secretion of HBsAg, HBeAg, and HBV DNA from HepG2.2.15 cells. At the same time, AFN preparation promoted not only the activation rate of NF-κB positive cells, but also antiviral GSH-PX enzyme function. In conclusion, nano-encapsulation of the flavonoids extract of Artemisia rupestris L. showed an enhanced anti-HBV effect in vitro compared to the original total flavonoids extract (without nano-encapsulation); therefore, nano-encapsulation has great potential for the development of a novel antiviral herbal medicine preparation with improved efficacy.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Molecular Medicine and Innovative Therapeutics
Publisher: TMR Publishing Group Limited
URI: http://researchrepository.murdoch.edu.au/id/eprint/65890
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