Analysis of the SNP loci around transcription start sites related to goat fecundity trait base on whole genome resequencing

Zhang, R-Q, Lai, F-N, Wang, J-J, Zhai, H-L, Zhao, Y., Sun, Y-J, Min, L-J and Shen, W. (2018) Analysis of the SNP loci around transcription start sites related to goat fecundity trait base on whole genome resequencing. Gene, 643 . pp. 1-6.

Abstract

Genome flanking regions surrounding transcription start sites (TSSs) are critical for the regulation of gene expression, containing many translational regulatory elements. To investigate whether critical single nucleotide polymorphisms (SNPs) exist around TSSs in the dairy goat genome, we performed high throughput DNA sequencing to compare two dairy goat groups with discrepant litter sizes. After genome mapping, SNP calling, and annotation, we screened the SNPs within 2 kb scales surrounding annotated TSSs in high fecundity (HF) and low fecundity (LF) groups, respectively. We attempted to identify distinct SNPs and motifs near the TSSs in both groups. The SNPs near the TSSs most were consistent; 318 new SNPs were uncovered in the HF group, of which 305 were heterozygote SNPs, 13 were homozygote SNPs, and majority of which were distributed on chromosome 2 and 29. After validation by Sanger sequencing we found that a SNP in CHI16: 27612330 C > A in the PSEN2

Item Type:	Journal Article
Publisher:	Elsevier BV
Copyright:	© 2017 Elsevier B.V.
URI:	http://researchrepository.murdoch.edu.au/id/eprint/65292

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