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Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea (Pisum sativum L.)

Li, G., Liu, R., Xu, R., Varshney, R.K.ORCID: 0000-0002-4562-9131, Ding, H., Li, M., Yan, X., Huang, S., Li, J., Wang, D., Ji, Y., Wang, C., He, J., Luo, Y., Gao, S., Wei, P., Zong, X. and Yang, T. (2022) Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea (Pisum sativum L.). The Crop Journal . In Press.

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Pea (Pisum sativum L.) is an annual cool-season legume crop. Owing to its role in sustainable agriculture as both a rotation and a cash crop, its global market is expanding and increased production is urgently needed. For both technical and regulatory reasons, neither conventional nor transgenic breeding techniques can keep pace with the demand for increased production. In answer to this challenge, CRISPR/Cas9 genome editing technology has been gaining traction in plant biology and crop breeding in recent years. However, there are currently no reports of the successful application of the CRISPR/Cas9 genome editing technology in pea. We developed a transient transformation system of hairy roots, mediated by Agrobacterium rhizogenes strain K599, to validate the efficiency of a CRISPR/Cas9 system. Further optimization resulted in an efficient vector, PsU6.3-tRNA-PsPDS3-en35S-PsCas9. We used this optimized CRISPR/Cas9 system to edit the pea phytoene desaturase (PsPDS) gene, causing albinism, by Agrobacterium-mediated genetic transformation. This is the first report of successful generation of gene-edited pea plants by this route.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Crop and Food Innovation
Western Australian State Agricultural Biotechnology Centre
Publisher: Elsevier B.V.
Copyright: © 2022 Crop Science Society of China and Institute of Crop Science, CAAS.
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