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Assessing the activity of antimicrobial peptides against common marine bacteria located in rotifer (Brachionus plicatilis) cultures

Woods, C., Woolley, L., Partridge, G., Chen, M., Haney, E.F., Hancock, R.E.W., Buller, N. and Currie, A. (2022) Assessing the activity of antimicrobial peptides against common marine bacteria located in rotifer (Brachionus plicatilis) cultures. Probiotics and Antimicrobial Proteins .

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Free to read: https://doi.org/10.1007/s12602-022-09928-2
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Abstract

Rotifers are used as the first feed for marine fish larvae and are grown in large cultures that have high loads of organic matter and heterotrophic bacteria; these bacteria are passed on to the developing fish larvae and can potentially lead to bacterial infections. A modified minimum inhibitory concentration (MIC) protocol for antimicrobial peptides was used to determine the potency of ten antimicrobial peptides (AMPs) in artificial seawater relevant to a rotifer culture (salinity of 25‰) against common marine pathogens. All of the AMPs had antimicrobial activity against the bacterial isolates when the salt concentration was approximately zero. However, in high salt concentrations, the majority of the AMPs had an MIC value greater than 65 µg mL−1 in artificial seawater (25‰). The only exceptions were 2009 (32.5 µg mL−1) and 3002 (32.5 µg mL−1) against Vibrio rotiferianus and Tenacibaculum discolor, respectively. The selected synthetic AMPs were not effective at reducing the bacterial load in brackish salt concentrations of a typical commercial rotifer culture (25‰).

Item Type: Journal Article
Murdoch Affiliation(s): College of Science, Health, Engineering and Education
Centre for Molecular Medicine and Innovative Therapeutics
Publisher: Springer New York LLC
Copyright: © 2022 The Authors.
URI: http://researchrepository.murdoch.edu.au/id/eprint/65184
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