Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Plasma lipid profiles change with increasing numbers of mild traumatic brain injuries in rats

Anyaegbu, C.C., Szemray, H., Hellewell, S.C., Lawler, N.G., Leggett, K., Bartlett, C., Lins, B., McGonigle, T., Papini, M., Anderton, R.S., Whiley, L.ORCID: 0000-0002-9088-4799 and Fitzgerald, M. (2022) Plasma lipid profiles change with increasing numbers of mild traumatic brain injuries in rats. Metabolites, 12 (4). Article 322.

PDF - Published Version
Available under License Creative Commons Attribution.

Download (954kB) | Preview
Free to read:
*No subscription required


Mild traumatic brain injury (mTBI) causes structural, cellular and biochemical alterations which are difficult to detect in the brain and may persist chronically following single or repeated injury. Lipids are abundant in the brain and readily cross the blood-brain barrier, suggesting that lipidomic analysis of blood samples may provide valuable insight into the neuropathological state. This study used liquid chromatography-mass spectrometry (LC-MS) to examine plasma lipid concentrations at 11 days following sham (no injury), one (1×) or two (2×) mTBI in rats. Eighteen lipid species were identified that distinguished between sham, 1× and 2× mTBI. Three distinct patterns were found: (1) lipids that were altered significantly in concentration after either 1× or 2× F mTBI: cholesterol ester CE (14:0) (increased), phosphoserine PS (14:0/18:2) and hexosylceramide HCER (d18:0/26:0) (decreased), phosphoinositol PI(16:0/18:2) (increased with 1×, decreased with 2× mTBI); (2) lipids that were altered in response to 1× mTBI only: free fatty acid FFA (18:3 and 20:3) (increased); (3) lipids that were altered in response to 2× mTBI only: HCER (22:0), phosphoethanolamine PE (P-18:1/20:4 and P-18:0/20:1) (increased), lysophosphatidylethanolamine LPE (20:1), phosphocholine PC (20:0/22:4), PI (18:1/18:2 and 20:0/18:2) (decreased). These findings suggest that increasing numbers of mTBI induce a range of changes dependent upon the lipid species, which likely reflect a balance of damage and reparative responses.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Computational and Systems Medicine
Health Futures Institute
Publisher: MDPI
Copyright: © 2022 by the authors
Item Control Page Item Control Page


Downloads per month over past year