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Characterisation of B cell subsets in the blood and lung of patients with idiopathic pulmonary fibrosis

Prêle, C., Lucas, A., Barrett, L., Baltic, S., Fear, M., Knight, D., Laurent, G., McAnulty, R., Mutsaers, S. and Hoyne, G. (2018) Characterisation of B cell subsets in the blood and lung of patients with idiopathic pulmonary fibrosis. Respirology, 23 (Supp. 1).

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Idiopathic pulmonary fibrosis is the most common of the idiopathic interstitial pneumonias and is typically associated with prominent lymphoid aggregates of CD3+ T cells and CD20+ B cells within the lung tissue that are located near sites of active fibrosis. The presence of lymphoid aggregates outside of primary and secondary lymphoid tissues is a prominent feature of many autoimmune diseases and the presence of lymphoid foci can precede the onset of clinical disease. We have examined the B cell profile of IPF patients and aged matched healthy controls using multicolour flow cytometry and analysed the serum for the presence of B cell cytokines BAFF and April, and CXCL13 a chemokine that promotes B cell migration to lung tissue. In contrast to healthy controls IPF patients show an accumulation of plasma B cells in the peripheral blood and a subset of patients show a rise in CD5+ CD23+ transitional B cells. Immunohistochemical staining of lung tissue from IPF patients revealed synchronous accumulation of CD138+ plasma cells and CD5+ B cells within the lymphoid aggregates of IPF patients. IPF patients showed a trend toward increased serum levels of BAFF, April and CXCL13. The presence of plasma cells and CD5+ B cells in the blood and lung tissue of IPF patients raises the important question as to the role of B cells in IPF disease pathogenesis.

Grant Support
This work is funded by NHMRC Project Grant GNT1067511 and British Lung Foundation Grant PPRG15-10.

Item Type: Journal Article
Publisher: Wiley-Blackwell
Copyright: © 2018 Asian Pacific Society of Respirology.
Other Information: Poster presentation given @ The Australia & New Zealand Society of Respiratory Science and The Thoracic Society of Australia and New Zealand (ANZSRS/TSANZ) Annual Scientific Meeting, Adelaide, Australia, 23–27 March 2018
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