Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Food waste digestate as a source of nitrogen for the cultivation of Dunaliella salina: Influence on growth and carotenogenesis under hyper osmotic stress

Chuka-ogwude, D., Nafisi, M., Taher, H., Ogbonna, J.C. and Moheimani, N.R.ORCID: 0000-0003-2310-4147 (2022) Food waste digestate as a source of nitrogen for the cultivation of Dunaliella salina: Influence on growth and carotenogenesis under hyper osmotic stress. Journal of Applied Phycology, 34 . pp. 101-112.

Link to Published Version: https://doi.org/10.1007/s10811-021-02663-1
*Subscription may be required

Abstract

Dunaliella salina is well-known for its ability to accumulate high amounts of carotenoids when cultivated under high salinity and high irradiance. However, as with all microalgae, cultivation processes are expensive when synthetic fertilizers are used for cultivation. In this study, we assessed the feasibility of utilizing anaerobic digestate of food waste as a source of nitrogen for the cultivation. Dunaliella salina MUR 8 was cultivated in F-medium with partial to complete replacement of the nitrogen (0–100% digestate nitrogen) source under high salinity (150–250‰) conditions to study both cell growth and carotenogenesis. Further investigation of the physiological response of D. salina was performed under varying irradiance and temperature levels. Maximum growth rate of 0.141 ± 0.022 day−1 and carotenoid content of 3.53 ± 0.47 mg g−1 were achieved at 150‰ salinity and under mild irradiance of 180 µmol photons m−2 s−1, in medium where nitrogen was completely replaced with food waste digestate nitrogen. Higher irradiance above 400 µmol photons m−2 s−1 and higher salinity had combined negative effects on growth and carotenogenesis, reducing growth rate and carotenoid content to 0.047 ± 0.01 day−1 and 1.797 ± 0.37 mg g−1, respectively, due to the NH3–N species present in the digestate. However, under increased irradiation and temperature, in comparison with cultures grown in synthetic medium with nitrate salts as nitrogen source, there was no significant difference in biomass productivity when the strain was cultivated using food waste digestate as sole nitrogen source.

Item Type: Journal Article
Murdoch Affiliation(s): Environmental and Conservation Sciences
Harry Butler Institute
Centre for Water, Energy and Waste
Algae R&D Centre
Publisher: Springer
Copyright: © The Author(s), under exclusive licence to Springer Nature B.V. 2021
URI: http://researchrepository.murdoch.edu.au/id/eprint/63701
Item Control Page Item Control Page