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Analysis of Small RNA Sequencing Data in Plants

Garg, V. and Varshney, R.K.ORCID: 0000-0002-4562-9131 (2022) Analysis of Small RNA Sequencing Data in Plants. Methods in molecular biology, 2443 . pp. 497-509.

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Over the past decades, next-generation sequencing (NGS) has been employed extensively for investigating the regulatory mechanisms of small RNAs. Several bioinformatics tools are available for aiding biologists to extract meaningful information from enormous amounts of data generated by NGS platforms. This chapter describes a detailed methodology for analyzing small RNA sequencing data using different open source tools. We elaborate on various steps involved in analysis, from processing the raw sequencing reads to identifying miRNAs, their targets, and differential expression studies.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Crop and Food Innovation
Food Futures Institute
Western Australian State Agricultural Biotechnology Centre
Publisher: Humana Press, Inc.
Copyright: © 2022 The Authors.
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