Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Transcriptional analysis of barley genes in response to drought stress at the reproductive growth stage using affymetrix Barley 1 genechip

Guo, P., Baum, M., Li, R-H, Grando, S., Varshney, R.K.ORCID: 0000-0002-4562-9131, Graner, A. and Ceccarelli, J.V. (2007) Transcriptional analysis of barley genes in response to drought stress at the reproductive growth stage using affymetrix Barley 1 genechip. Journal of Guangzhou University, 6 (5). pp. 32-36.

Abstract

Drought tolerance is a key trait for increasing and stabilizing barley productivity in dry areas. The understanding of the molecular mechanism of drought tolerance during the reproductive stages may facilitate the identification of novel genes controlling traits associated with drought tolerance for biotechnology-assisted genetic improvement of barley. In this study, the 22K Affymetrix Genechip Barley 1 array was used to monitor changes in the transcription levels under drought stress of two cultivars, Tadmor and WI2291, drought tolerant and sensitive, respectively. The preliminary results revealed that 77 genes showed significant differences in transcript abundance in both varieties under drought stress. The list includes many drought-responsive genes that were identified in previous studies. The genes showing similarity to proteins of known function were classified into 14 different functional categories. These 77 genes were expressed in both varieties when plants were exposed to water stress;therefore, they are likely to be genes responsive to drought stress and not important in drought tolerance. When gene expression under drought stress was further analyzed, 372 genes were identified to be significantly differentially expressed between two varieties. Those genes with known function were classified into 15 different functional categories in biological process. Some of these genes are known genes related to drought tolerance, while the others are unknown function or novel genes,which may be involved in drought tolerance. These results could provide new insights to elucidate the mechanism for drought tolerance in barley during the heading stage.

Item Type: Journal Article
Publisher: guang zhou da xue xue bao bian ji bu
Publisher's Website: https://www.oriprobe.com/journals/gzdxxb-zkb.html
URI: http://researchrepository.murdoch.edu.au/id/eprint/63601
Item Control Page Item Control Page