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G4009.07.03: Marker-assisted backcrossing (MABC) for drought tolerance in chickpea-students for analysis of drought tolerance in chickpea (TLI-Kenyan student)

Varshney, R.K.ORCID: 0000-0002-4562-9131, Kimurto, P., Mulwa, R., Gaur, P. and Thudi, M. (2011) G4009.07.03: Marker-assisted backcrossing (MABC) for drought tolerance in chickpea-students for analysis of drought tolerance in chickpea (TLI-Kenyan student). Project Updates. CGIAR Generation Challenge Programme .

Abstract

Chickpea is an important grain legume in South Asia (SA) and sub-Saharan Africa (SSA) especially in eastern and southern Africa. Drought is a major abiotic constraint affecting production of chickpea worldwide. Until recently, breeding efforts to improve drought tolerance have been hindered due to its quantitative genetic basis and poor understanding of the physiological basis of yield in water-limited conditions. Recently at ICRISAT, superior lines with improved drought tolerance have been identified and a large number of molecular markers developed. A genomic region harbouring several QTLs for root traits contributing up to 30% phenotypic variation was identified on linkage group 5 of the chickpea genome under Tropical Legume I (TLI) project. The major goal of this project is to enhance the drought tolerance of elite Kenyan genotypes using the marker-assisted back crossing (MABC) approach. Based on marker polymorphism assay on ten agronomically elite Kenyan chickpea cultivars and ICC 4958 (a drought tolerant variety from India), ICCV 95423, an elite cultivar from Kenya was selected as a recurrent parent. In October 2009, the first cross of the recurrent and donor parent was made to obtain F1 .In April 2010, on identification of heterozygous F1s, backcrossing to the recurrent parent to obtain BC1F1 was done. In July 2010, after foreground selection with markers flanking to the QTL region, selected BC1F1 plants were backcrossed to the recurrent parent to obtain BC2F1. In the main 2010 crop season (Oct 2010- Feb 2011), selected BC2F1 plants after foreground selection were backcrossed to the recurrent parent and BC3F1 seeds were harvested. These seeds will be used for selfing in the 2011 off-season and subsequently BC3F2 seeds will be used for seed multiplication in the main 2011 crop season. In parallel, selected BC3F2 lines will be phenotyped for root traits to confirm the introgression of the QTL region. Selected lines will be eventually tested in field conditions in Kenya and India to identify the best performing lines for there possible release as drought tolerant varieties.

Item Type: Others
Publisher: CIMMYT
Publisher's Website: https://www.generationcp.org/
URI: http://researchrepository.murdoch.edu.au/id/eprint/63329
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