Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Novel SSR markers from BAC-End Sequences, DArT Arrays and a comprehensive genetic map with 1,291 marker loci for Chickpea (Cicer arietinum L.)

Hansson, B., Thudi, M., Bohra, A., Nayak, S.N., Varghese, N., Shah, T.M., Penmetsa, R.V., Thirunavukkarasu, N., Gudipati, S., Gaur, P.M., Kulwal, P.L., Upadhyaya, H.D., Kavikishor, P.B., Winter, P., Kahl, G., Town, C.D., Kilian, A., Cook, D.R. and Varshney, R.K.ORCID: 0000-0002-4562-9131 (2011) Novel SSR markers from BAC-End Sequences, DArT Arrays and a comprehensive genetic map with 1,291 marker loci for Chickpea (Cicer arietinum L.). PLoS ONE, 6 (11). Art. e27275.

[img]
Preview
PDF - Published Version
Download (1MB) | Preview
Free to read: https://doi.org/10.1371/journal.pone.0027275
*No subscription required

Abstract

Chickpea (Cicer arietinum L.) is the third most important cool season food legume, cultivated in arid and semi-arid regions of the world. The goal of this study was to develop novel molecular markers such as microsatellite or simple sequence repeat (SSR) markers from bacterial artificial chromosome (BAC)-end sequences (BESs) and diversity arrays technology (DArT) markers, and to construct a high-density genetic map based on recombinant inbred line (RIL) population ICC 4958 (C. arietinum)×PI 489777 (C. reticulatum). A BAC-library comprising 55,680 clones was constructed and 46,270 BESs were generated. Mining of these BESs provided 6,845 SSRs, and primer pairs were designed for 1,344 SSRs. In parallel, DArT arrays with ca. 15,000 clones were developed, and 5,397 clones were found polymorphic among 94 genotypes tested. Screening of newly developed BES-SSR markers and DArT arrays on the parental genotypes of the RIL mapping population showed polymorphism with 253 BES-SSR markers and 675 DArT markers. Segregation data obtained for these polymorphic markers and 494 markers data compiled from published reports or collaborators were used for constructing the genetic map. As a result, a comprehensive genetic map comprising 1,291 markers on eight linkage groups (LGs) spanning a total of 845.56 cM distance was developed (http://cmap.icrisat.ac.in/cmap/sm/cp/thudi/). The number of markers per linkage group ranged from 68 (LG 8) to 218 (LG 3) with an average inter-marker distance of 0.65 cM. While the developed resource of molecular markers will be useful for genetic diversity, genetic mapping and molecular breeding applications, the comprehensive genetic map with integrated BES-SSR markers will facilitate its anchoring to the physical map (under construction) to accelerate map-based cloning of genes in chickpea and comparative genome evolution studies in legumes.

Item Type: Journal Article
Publisher: Public Library of Science
Copyright: © 2011 Thudi et al.
URI: http://researchrepository.murdoch.edu.au/id/eprint/63308
Item Control Page Item Control Page

Downloads

Downloads per month over past year