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Peanut seed coat acts as a physical and biochemical barrier against Aspergillus flavus infection

Commey, L., Tengey, T.K., Cobos, C.J., Dampanaboina, L., Dhillon, K.K., Pandey, M.K., Sudini, H.K., Falalou, H., Varshney, R.K.ORCID: 0000-0002-4562-9131, Burow, M.D. and Mendu, V. (2021) Peanut seed coat acts as a physical and biochemical barrier against Aspergillus flavus infection. Journal of Fungi, 7 (12). Art. 1000.

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Abstract

Aflatoxin contamination is a global menace that adversely affects food crops and human health. Peanut seed coat is the outer layer protecting the cotyledon both at pre- and post-harvest stages from biotic and abiotic stresses. The aim of the present study is to investigate the role of seed coat against A. flavus infection. In-vitro seed colonization (IVSC) with and without seed coat showed that the seed coat acts as a physical barrier, and the developmental series of peanut seed coat showed the formation of a robust multilayered protective seed coat. Radial growth bioassay revealed that both insoluble and soluble seed coat extracts from 55-437 line (resistant) showed higher A. flavus inhibition compared to TMV-2 line (susceptible). Further analysis of seed coat biochemicals showed that hydroxycinnamic and hydroxybenzoic acid derivatives are the predominant phenolic compounds, and addition of these compounds to the media inhibited A. flavus growth. Gene expression analysis showed that genes involved in lignin monomer, proanthocyanidin, and flavonoid biosynthesis are highly abundant in 55-437 compared to TMV-2 seed coats. Overall, the present study showed that the seed coat acts as a physical and biochemical barrier against A. flavus infection and its potential use in mitigating the aflatoxin contamination.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Crop and Food Innovation
Western Australian State Agricultural Biotechnology Centre
Publisher: MDPI
Copyright: © 2021 The Authors.
URI: http://researchrepository.murdoch.edu.au/id/eprint/63159
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