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Stability of ex vivo coagulation factor activity in never‐frozen and thawed refrigerated canine plasma stored for 42 days

Chee, W., Sharp, C.R.ORCID: 0000-0002-1797-9783, Boyd, C.J.ORCID: 0000-0003-1361-2148, Claus, M.A.ORCID: 0000-0003-1529-1480 and Smart, L.ORCID: 0000-0003-4776-2849 (2021) Stability of ex vivo coagulation factor activity in never‐frozen and thawed refrigerated canine plasma stored for 42 days. Journal of Veterinary Emergency and Critical Care .

Link to Published Version: https://doi.org/10.1111/vec.13152
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Abstract

Objective

The primary objective of this study was to document coagulation factor activity in canine “NEVER-FROZEN” and “THAWED” refrigerated plasma for the purposes of defining recommended expiration dates. We hypothesized that NEVER-FROZEN and THAWED refrigerated plasma would maintain >50% activity of coagulation factors V (FV), VII (FVII), VIII (FVIII), IX (FIX), X (FX), and von Willebrand factor antigen (vWF) and a concentration of fibrinogen above the lower bound of the reference interval (>0.982 g/L) for greater than 14 days but less than 42 days.

Design

Prospective laboratory-based study.

Setting

University teaching hospital blood bank.

Animals

Ten canine plasma units derived from healthy client-owned blood donors.

Interventions

Serial sampling (days 0, 1, 3, 5, 7, 10, 14, 17, 21, 24, 28, 32, 35, 39, 42) from NEVER-FROZEN and THAWED refrigerated canine plasma units was conducted for measurement of activities of FV, FVII, FVIII, FIX, FX, vWF, and fibrinogen concentrations using the ACL TOP 300. Plasma was defined as “suitable for transfusion” at a given time point if the entire 95% confidence interval for each factor was above 50% activity and above a fibrinogen concentration of 0.982 g/L.

Measurements and main results

The lower bounds of the FVIII and vWF confidence intervals were above 50% up to and including day 32 for NEVER-FROZEN refrigerated plasma and day 28 for THAWED refrigerated plasma. Confidence intervals for FV, FVII, FIX, and FX remained above 50% activity at all time points. The lower bound of the fibrinogen concentration was <0.982 g/L on day 39 for NEVER-FROZEN refrigerated plasma and on day 35 for THAWED refrigerated plasma.

Conclusions

Refrigerated canine plasma from these 10 dogs retained coagulation factor activity above the limit that we defined as suitable for transfusion for up to 32 days when NEVER-FROZEN and 28 days when THAWED. Further studies should evaluate the clinical outcomes and effects on coagulation factor activity of dogs receiving refrigerated plasma transfusions.

Item Type: Journal Article
Murdoch Affiliation(s): Veterinary Medicine
Publisher: Blackwell Publishing Inc.
Copyright: © 2021Veterinary Emergency and Critical Care Society
URI: http://researchrepository.murdoch.edu.au/id/eprint/62969
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