Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Effectiveness of augmentative biological control of Streptomyces griseorubens UAE2 depends on 1-Aminocyclopropane-1-Carboxylic Acid deaminase activity against Neoscytalidium dimidiatum

Al Hamad, B.M., Al Raish, S.M., Ramadan, G.A., Saeed, E.E., Alameri, S.S.A., Al Senaani, S.S., AbuQamar, S.F. and El-Tarabily, K.A. (2021) Effectiveness of augmentative biological control of Streptomyces griseorubens UAE2 depends on 1-Aminocyclopropane-1-Carboxylic Acid deaminase activity against Neoscytalidium dimidiatum. Journal of Fungi, 7 (11). Art. 885.

[img]
Preview
PDF - Published Version
Download (3MB) | Preview
Free to read: https://doi.org/10.3390/jof7110885
*No subscription required

Abstract

To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong in vitro inhibitory effects against Neoscytalidiumdimidiatum. Streptomyces griseorubens UAE2 and Streptomyces wuyuanensis UAE1 had the ability to produce antifungal compounds and cell-wall-degrading enzymes (CWDEs). Only S. griseorubens, however, restored the activity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD). In vivo apple fruit bioassay showed that lesion development was successfully constrained by either isolates on fruits inoculated with N. dimidiatum. In our greenhouse and container nursery experiments, S. griseorubens showed almost complete suppression of disease symptoms. This was evident when the preventive treatment of S. griseorubens significantly (p < 0.05) reduced the numbers of conidia of N. dimidiatum and defoliated leaves of royal poinciana seedlings to lesser levels than when S. wuyuanensis was applied, but comparable to control treatments (no pathogen). The disease management of stem canker was also associated with significant (p < 0.05) decreases in ACC levels in royal poinciana stems when S. griseorubens was applied compared to the non-ACCD-producing S. wuyuanensis. This study is the first to report the superiority of antagonistic actinobacteria to enhance their effectiveness as BCAs not only for producing antifungal metabolites and CWDEs but also for secreting ACCD.

Item Type: Journal Article
Murdoch Affiliation(s): Harry Butler Institute
Publisher: MDPI
Copyright: © 2021 The Authors.
URI: http://researchrepository.murdoch.edu.au/id/eprint/62810
Item Control Page Item Control Page

Downloads

Downloads per month over past year