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MT137 Comparative analyses of PBMC transcriptome profiles between German Landrace and Pietrain pigs following PRRSV vaccination

Islam, Md. A., Pröll, M.J., Rony, S.A., Große-Brinkhaus, C., Uddin, M.J., Tesfaye, D., Hoelker, M., Tholen, E., Schellander, K. and Neuhoff, C. (2017) MT137 Comparative analyses of PBMC transcriptome profiles between German Landrace and Pietrain pigs following PRRSV vaccination. In: 36th International Society of Animal Genetics (ISAG) Conference, 16 - 21 July 2017, Dublin, Ireland.

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is a devastating viral disease affecting swine industry worldwide. The innate immune response to PRRS virus (PRRSV) infection varies among pig breeds. Therefore, the current study aimed to investigate the breed difference in innate immune response to PRRSV vaccination between German Landrace (DL) and Pietrain (Pi) pigs. For this we used a total of 12 Affymetrix GeneChip porcine gene 1.0 ST array for transcriptome profiling of peripheral blood mononuclear cells (PBMCs) collected before (0h) and 24 h after PRRSV vaccination from three female piglets of four weeks age. With FDR < 0.01 and log2 fold change 1.5 as cutoff criteria, 4269 transcripts were found to be differentially expressed among four contrast pairs tested (DL-24h v. DL-0h, Pi-24h v. Pi-0h, DL-0h v. Pi-0h and DL-24h v. Pi-24h). The number of vaccine induced differentially expressed genes (DEG) was much higher (DL-0h v. DL-24h, DEG = 2459) in DL pigs than that of Pi pigs (Pi-24h v. Pi-0h, DEG = 291). Before vaccination, 3255 genes showed differential expression between DL and Pi (DL-0h v. Pi-0h) which indicated the genetic variation between two breeds. After 24 h of PRRSV vaccination, 1046 genes were overexpressed in DL pig compared to Pi (DL-24h v. Pi-24h) which indicated the breed differences in vaccine responsiveness. The top most biological pathways enriched by differentially expressed genes are linked to immune response function e.g.: cytokine signalling in immune system, GPCR signalling, JAK-STAT signalling or interferon signalling. The network enrichment analysis identified STAT1, MMS19, RPA2, BAD, UCHL5 and APC as potential regulatory genes for the functional network of PRRSV vaccine response specific for DL and FOXO3, IRF2, ADRBK1, MTOR and EIF3I genes for Pi pigs. SLC9A2, BAG3, RRS1, DCTN3, BUD31, ARPC1B and ATP5J2 were found to be the most potential hubs of the shared network of transcriptome response in PBMCs of both DL and Pi pigs. In conclusion, DL pigs differ greatly from that of Pi in terms of PBMCs transcriptome profiles after PRRSV vaccination.

Item Type: Conference Item
URI: http://researchrepository.murdoch.edu.au/id/eprint/62724
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