Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Variant discovery in the sheepmeat odour and flavour in javanese fat tailed sheep using RNA sequencing

Abuzahra, M.A.M., Jakaria, ., Listyarini, K., Furqon, A., Sumantri, C., Uddin, M.J. and Gunawan, A. (2018) Variant discovery in the sheepmeat odour and flavour in javanese fat tailed sheep using RNA sequencing. IOP Conference Series: Earth and Environmental Science, 157 . Art. 012030.

[img]
Preview
PDF - Published Version
Download (615kB) | Preview
Free to read: https://doi.org/10.1088/1755-1315/157/1/012030
*No subscription required

Abstract

High-throughput RNA sequencing (RNA-Seq) reveals new challenges for the detection of transcriptome variants (SNPs) in different tissues and species. The aims of this study was to characterize a SNP discovery analysis in the sheep meat odour and flavour transcriptome using RNA-Seq. Six liver samples from divergent sheep meat odour and flavour were analyzed using the Illumina Genome Hiseq 2500 Analyzer. The SNP detection analysis revealed 142 SNPs in sheep meat samples, and a large number of those corresponded to differences between high and low sheep meat odour and flavour ovis genome assembly OAR v4.0. Among them, about 90.4% of genes had multiple polymorphisms within 12 genes (JAML, ANGPTL8, LOC101103463, SEPW1, SCN5A, LOC101113036, DOCK6, GTSE1, KIF12, KCTD17, KANK2, CYP2A6). Several of the SNPs (JAML, CYP2A6, SEPW1, and KIF12) found in this study could be included as suitable markers in genotyping platforms to perform association analyses in commercial populations and apply genomic selection protocols in the sheep meat production.

Item Type: Journal Article
Publisher: IOP Science
Other Information: Paper presented at 1st International Conference on Food Security and Sustainable Agriculture in The Tropics (IC-FSSAT) 24–25 October 2017, Sulawesi Selatan, Indonesia
URI: http://researchrepository.murdoch.edu.au/id/eprint/62719
Item Control Page Item Control Page

Downloads

Downloads per month over past year