Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Identification and association of polymorphism in THOC5 gene with fatty acid composition in Indonesian sheep

Listyarini, K., Sumantri, C., Rahayu, S., Uddin, M.J. and Gunawan, A. (2021) Identification and association of polymorphism in THOC5 gene with fatty acid composition in Indonesian sheep. IOP Conference Series: Earth and Environmental Science, 788 (1). Art. 012020.

[img]
Preview
PDF - Published Version
Download (299kB) | Preview
Free to read: https://doi.org/10.1088/1755-1315/788/1/012020
*No subscription required

Abstract

THO Complex 5 (THOC5) gene involves in lipid and fatty acid metabolism. The aim of this study was to analyse polymorphism of THOC5 gene and its association with fatty acid composition in sheep. A total of 120 rams at the age 12 month with the average body weight of 25–30 kg was used for identification of gene polymorphism using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP). 83 rams, representative from different genotypes of sheep were used for association study using a General Linear Model (GLM). The results identifed polymorphism in the THOC5 gene. The THOC5 gene showed two genotypes: CC and CT genotype. Association analysis revealed that THOC5 (g.68234589 C>T) was significantly (P<0.05) associated with fatty acid composition including unsaturated fatty acid: palmitoleic acid (C16:1), and saturated fatty acid: stearic acid (C18:0). The CC genotype was associated with higher level of unsaturated fatty acid and lower level of saturated fatty acid, while the CT genotype was vice versa. This result indicates that THOC5 gene (g.68234589 C>T) may contribute to fatty acid composition in sheep, as well as this polymorphism could be used as a candidate to select sheepmeat with high unsaturated fatty acid and low saturated fatty acid.

Item Type: Journal Article
Publisher: IOP Science
Other Information: Paper given at The 3rd International Conference of Animal Science and Technology 3-4 November 2020, Makassar, Indonesia
URI: http://researchrepository.murdoch.edu.au/id/eprint/62714
Item Control Page Item Control Page

Downloads

Downloads per month over past year