Genome-wide identification and validation of target genes associated with insecticide treatment of the green peach aphid, Myzus persicae

Iqbal, S., Fosu-Nyarko, J., Brigg, F. and Jones, M.G.K.ORCID: 0000-0001-5002-0227 Genome-wide identification and validation of target genes associated with insecticide treatment of the green peach aphid, Myzus persicae. Methods in Molecular Biology, 2360 . pp. 119-138.

Abstract

Next-generation sequencing and analyses of whole-genome transcripts can be used to identify genes and potential mechanisms that may be responsible for the development of resistance to insecticides. Such genes can be identified by isolating and sequencing high-quality messenger RNA and identifying differentially expressed genes (DEGs), and gene variants from insecticide-treated and untreated colonies of the Green peach aphid (GPA) or resistant and susceptible GPA populations. Datasets generated would reveal a set of genes whose expression may be associated with the insecticide treatment. The DEGs can then be validated using quantitative PCR assays.

Item Type:	Journal Article
Murdoch Affiliation(s):	College of Science, Health, Engineering and Education Western Australian State Agricultural Biotechnology Centre
Publisher:	Springer Nature
Copyright:	© 2022. The Author(s), under exclusive license to Springer Science+Business Media
URI:	http://researchrepository.murdoch.edu.au/id/eprint/62332

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