Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Association study and expression analysis of stearoyl Co-A desaturase as a candidate gene for fatty acid composition in Indonesian crossbred chickens

Gunawan, A., Nurajizah, E.S., Listyarini, K., Furqon, A., Bilyaro, W., Sumantri, C., Jakaria, ., Akter, S.H. and Uddin, M.J. (2018) Association study and expression analysis of stearoyl Co-A desaturase as a candidate gene for fatty acid composition in Indonesian crossbred chickens. International Journal of Poultry Science, 17 (7). pp. 348-355.

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (610kB) | Preview
Free to read: https://doi.org/10.3923/ijps.2018.348.355
*No subscription required

Abstract

Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens.

Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken.

Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens.

Conclusion:These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA.

Item Type: Journal Article
Publisher: Asian Network for Scientific Information (ANSINET)
Copyright: © 2018 Asep Gunawan et al.
URI: http://researchrepository.murdoch.edu.au/id/eprint/62329
Item Control Page Item Control Page

Downloads

Downloads per month over past year