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Real-time fluorometric isothermal LAMP assay for detection of Chlamydia pecorum in rapidly processed ovine abortion samples: A veterinary practitioner’s perspective

Clune, T., Anstey, S., Kasimov, V., Jacobson, C.ORCID: 0000-0001-9427-1941 and Jelocnik, M. (2021) Real-time fluorometric isothermal LAMP assay for detection of Chlamydia pecorum in rapidly processed ovine abortion samples: A veterinary practitioner’s perspective. Pathogens, 10 (9). Article 1157.

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Abstract

Traditional methods of detecting Chlamydia pecorum in tissue samples such as polymerase chain reaction or cell culture are laborious and costly. We evaluated the use of a previously developed C. pecorum LAMP assay using minimally processed ovine samples. Cotyledon (n = 16), foetal liver (n = 22), foetal lung (n = 2), and vaginal (n = 6) swabs, in addition to cotyledon (n = 6) and foetal liver (n = 8) tissue samples, were rapidly processed and used for LAMP testing without DNA extraction. Overall, LAMP test results were highly congruent with the in-house reference qPCR, with 80.43% (37/46; 72.73% positive agreement (PA); 84.75% negative agreement (NA)) overall agreeance for swab samples, and 85.71% (12/14; 80% PA; 88.89% NA) overall agreeance for tissue samples. Out of the 11 total discrepant results, discrepancy was mainly observed in samples (n = 10) with less than 100 copies/µL C. pecorum DNA. While sensitivity could be improved, the simplicity, low cost, and accuracy of detection makes this test amenable for use at point-of-care for detecting C. pecorum in sheep.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Animal Production and Health
Publisher: MDPI
Copyright: © 2021 by the authors
URI: http://researchrepository.murdoch.edu.au/id/eprint/62248
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