Real-time fluorometric isothermal LAMP assay for detection of Chlamydia pecorum in rapidly processed ovine abortion samples: A veterinary practitioner’s perspective

Clune, T., Anstey, S., Kasimov, V., Jacobson, C.ORCID: 0000-0001-9427-1941 and Jelocnik, M. (2021) Real-time fluorometric isothermal LAMP assay for detection of Chlamydia pecorum in rapidly processed ovine abortion samples: A veterinary practitioner’s perspective. Pathogens, 10 (9). Article 1157.

Abstract

Traditional methods of detecting Chlamydia pecorum in tissue samples such as polymerase chain reaction or cell culture are laborious and costly. We evaluated the use of a previously developed C. pecorum LAMP assay using minimally processed ovine samples. Cotyledon (n = 16), foetal liver (n = 22), foetal lung (n = 2), and vaginal (n = 6) swabs, in addition to cotyledon (n = 6) and foetal liver (n = 8) tissue samples, were rapidly processed and used for LAMP testing without DNA extraction. Overall, LAMP test results were highly congruent with the in-house reference qPCR, with 80.43% (37/46; 72.73% positive agreement (PA); 84.75% negative agreement (NA)) overall agreeance for swab samples, and 85.71% (12/14; 80% PA; 88.89% NA) overall agreeance for tissue samples. Out of the 11 total discrepant results, discrepancy was mainly observed in samples (n = 10) with less than 100 copies/µL C. pecorum DNA. While sensitivity could be improved, the simplicity, low cost, and accuracy of detection makes this test amenable for use at point-of-care for detecting C. pecorum in sheep.

Item Type:	Journal Article
Murdoch Affiliation(s):	Centre for Animal Production and Health
Publisher:	MDPI
Copyright:	© 2021 by the authors
URI:	http://researchrepository.murdoch.edu.au/id/eprint/62248

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