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Fast forward genetic mapping provide candidate genes for resistance to fusarium wilt and sterility mosaic disease resistance in pigeonpea (Cajanus cajan L. Millsp.)

Singh, V.K., Khan, A.W., Takagi, H., Saxena, R.K., Kumar, V., Sharma, M., Sameer Kumar, C.V., Sinha, P., Chitikineni, A., Patil, S., Ghanta, A., Yamini, K.N., Parupalli, S., Muniswamy, S., Dharmaraj, P.S., Terauchi, R. and Varshney, R.K.ORCID: 0000-0002-4562-9131 (2014) Fast forward genetic mapping provide candidate genes for resistance to fusarium wilt and sterility mosaic disease resistance in pigeonpea (Cajanus cajan L. Millsp.). In: 6th International Food Legume Research Conference/7th International Conference on Legume Genetics and Genomics, 7 - 11 July 2014, TCU Place Saskatoon, Saskatchewan, Canada.

Abstract

Fast forward genetic mapping combines with whole genome sequencing (WGS) and bulked segregant analysis (BSA) approach was used to identify the candidate genes for Fusarium wilt (FW) and sterility mosaic disease (SMD) resistance in pigeonepa. To map the targeted genomic regions, F7 RILs developed by crossing ICPL 20096 (R) × ICP 332 (S) and segregating for FW and SMD resistance were phenotyped at two different locations in India. Based on the phenotyping, 16 RILs in each category were selected for development of resistant (R-Bulk) and susceptible bulks (S-Bulk). These two bulks along with resistant parent (ICPL 20096) were re-sequenced and generated ~19GB of 250 bp pair-end data with ~15× genome coverage. WGS data generated from R- and S- Bulks were aligned with resistant parent. As a result a total of 35,877 SNPs with SNP index of ≥3 were identified. Out of 35,877 SNPs only 4,139 (11.54%) SNPs were found homozygous. Based on the SNP index (0 for R-Bulk and 1 for S-Bulk) and SNP substitution effect three significant SNPs including two on CcLG07 and one on CcLG11 affects a total of four candidate genes. Functional annotation of these four genes indicated their role to initiate defence mechanism against fungal and viral diseases.

Item Type: Conference Item
Other Information: Poster presentation
URI: http://researchrepository.murdoch.edu.au/id/eprint/62231
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