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Genome sequence of mungbean and insights into evolution within Vigna species

Kang, Y.J., Kim, S.K., Kim, M.Y., Lestari, P., Kim, K.H., Ha, B.K., Jun, T.H., Hwang, W.J., Lee, T., Lee, J., Shim, S., Yoon, M.Y., Jang, Y.E., Han, K.S., Taeprayoon, P., Yoon, N., Somta, P., Tanya, P., Kim, K.S., Gwag, J-G, Moon, J.K., Lee, Y.H., Jackson, S.A., Schafleitner, R., Srinives, P., Varshney, R.K.ORCID: 0000-0002-4562-9131 and Lee, S.H. (2014) Genome sequence of mungbean and insights into evolution within Vigna species. In: 6th International Food Legume Research Conference/7th International Conference on Legume Genetics and Genomics, 7 - 11 July 2014, TCU Place Saskatoon, Saskatchewan, Canada.

Abstract

Mungbean (Vigna radiata) belongs to the subgenus Ceratotropisand is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis andto enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 Vigna accessions of 18 species and the protein sets of Glycine max, Lotus japonicus, Medicago truncatula, Cicer arietinum and Cajanus cajan, by Bayesian Markov chain Monte Carlo method using the loci containing the confident orthologs for all 27 accessions. The present assembly of V. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.

Item Type: Conference Item
Other Information: Poster presentation
URI: http://researchrepository.murdoch.edu.au/id/eprint/62227
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