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High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

Bayer, P.E., Ruperao, P., Mason, A.S., Stiller, J., Chan, C-K.K., Hayashi, S., Long, Y., Meng, J., Sutton, T., Visendi, P., Varshney, R.K.ORCID: 0000-0002-4562-9131, Batley, J. and Edwards, D. (2015) High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus. Theoretical and Applied Genetics, 128 (6). pp. 1039-1047.

Link to Published Version: https://doi.org/10.1007/s00122-015-2488-y
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Abstract

The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.

Item Type: Journal Article
Publisher: Springer Verlag
URI: http://researchrepository.murdoch.edu.au/id/eprint/61955
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