Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Major genetic discontinuity and novel toxigenic species in Clostridioides difficile taxonomy

Knight, D.R.ORCID: 0000-0002-9480-4733, Imwattana, K., Kullin, B., Guerrero-Araya, E., Paredes-Sabja, D., Didelot, X., Dingle, K.E., Eyre, D.W., Rodriguez, C. and Riley, T.V. (2021) Major genetic discontinuity and novel toxigenic species in Clostridioides difficile taxonomy. eLife, 10 . Art. e64325.

[img]
Preview
PDF - Published Version
Download (2MB) | Preview
Free to read: https://doi.org/10.7554/eLife.64325
*No subscription required

Abstract

Clostridioides difficile infection (CDI) remains an urgent global One Health threat. The genetic heterogeneity seen across C. difficile underscores its wide ecological versatility and has driven the significant changes in CDI epidemiology seen in the last 20 years. We analysed an international collection of over 12,000 C. difficile genomes spanning the eight currently defined phylogenetic clades. Through whole-genome average nucleotide identity, and pangenomic and Bayesian analyses, we identified major taxonomic incoherence with clear species boundaries for each of the recently described cryptic clades CI–III. The emergence of these three novel genomospecies predates clades C1–5 by millions of years, rewriting the global population structure of C. difficile specifically and taxonomy of the Peptostreptococcaceae in general. These genomospecies all show unique and highly divergent toxin gene architecture, advancing our understanding of the evolution of C. difficile and close relatives. Beyond the taxonomic ramifications, this work may impact the diagnosis of CDI.

Item Type: Journal Article
Murdoch Affiliation(s): Medical, Molecular and Forensic Sciences
Publisher: eLife Sciences Publications
Copyright: © 2021 Knight et al.
URI: http://researchrepository.murdoch.edu.au/id/eprint/61399
Item Control Page Item Control Page

Downloads

Downloads per month over past year