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Neuroprotective effects of hesperetin in mouse primary neurones are independent of CREB activation

Rainey-Smith, S., Schroetke, L-W, Bahia, P., Fahmi, A., Skilton, R., Spencer, J.P.E., Rice-Evans, C., Rattray, M. and Williams, R.J. (2008) Neuroprotective effects of hesperetin in mouse primary neurones are independent of CREB activation. Neuroscience Letters, 438 (1). pp. 29-33.

Link to Published Version: https://doi.org/10.1016/j.neulet.2008.04.056
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Abstract

Dietary flavonoids, including the citrus flavanone hesperetin, may have stimulatory effects on cytoprotective intracellular signalling pathways. In primary mouse cortical neurone cultures, but not SH-SY5Y human neuroblastoma cells or human primary dermal fibroblasts (Promocells), hesperetin (100–300 nM, 15 min) caused significant increases in the level of ERK1/2 phosphorylation, but did not increase CREB phosphorylation. Administration of hesperetin for 18 h did not alter gene expression driven by the cyclic AMP response element (CRE), assessed using a luciferase reporter system, but 300 nM hesperetin partially reversed staurosporine-induced cell death in primary neurones. Our data show that hesperetin is a neuroprotective compound at concentrations where antioxidant effects are unlikely to predominate. The effects of hesperetin are cell-type dependent and, unlike the flavanol (−)epicatechin, neuroprotection in vitro is not associated with enhanced CREB phosphorylation or CRE-mediated gene expression.

Item Type: Journal Article
Publisher: Elsevier
Copyright: © 2008 Elsevier Ireland Ltd.
URI: http://researchrepository.murdoch.edu.au/id/eprint/60904
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