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W565: Application of TILLING and BSA-Seq approach to map prominent main stem and leaf spot resistance in groundnut

Chitikineni, A., Wang, H., Agarwal, G., Khan, A.W., Pandey, M.K., Ozias-Akins, P., Holbrook, C., Varshney, R.K.ORCID: 0000-0002-4562-9131 and Guo, B. (2019) W565: Application of TILLING and BSA-Seq approach to map prominent main stem and leaf spot resistance in groundnut. In: Plant & Animal Genome Conference XXVII, 12 - 16 January 2019, San Diego, CA


Targeting Induced Local Lesions in Genomes (TILLING) is considered a powerful reverse genetics approach for functional genomics studies. However, because of availability of low-cost and high-throughput sequencing technology, it has become possible to sequence TILLING lines and identify SNPs associated with genes responsible for traits. One TILLING population has been developed in the “Tifrunner” genotype of groundnut, an economically important oilseed crop grown in tropical and warm temperate regions of the world. The TILLING population has shown phenotypic variation for several traits including resistance to leaf spots and the features of prominent main stem. A total of 25 lines comprising of 16 susceptible and 9 resistant lines for leaf spots, and 11 lines with presence and 14 lines with absence of the prominent main stem from the TILLING population were sequenced on Illumina HiSeq 2500 and a total of 745.8 Gb sequencing data has been generated. These sequence data are being analyzed to identify structural variations including SNPs and INDELs across the lines with Tifrunner. In parallel, two mapping populations from these TILLING lines namely T47-7 (resistant to leaf spots) x T33-3 (susceptible to leaf spots) and T90-1 (presence of stem) x T71-2 (absence of stem) are being developed. It is planned to phenotype the segregating progenies and also sequence the extreme bulksA of segregating progenies for these traits. We anticipate identification of candidate genes and SNPs for these important traits by deploying the BSA-Seq approach in groundnut in due course.

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