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Effects of potential probiotic Enterococcus casseliflavus (EC-001) on growth performance, immunity, and resistance to Aeromonas hydrophila infection in common Carp (Cyprinus carpio)

Akbari, H., Shekrabi, S.P.H., Soltani, M. and Mehrgan, M.S. (2021) Effects of potential probiotic Enterococcus casseliflavus (EC-001) on growth performance, immunity, and resistance to Aeromonas hydrophila infection in common Carp (Cyprinus carpio). Probiotics and Antimicrobial Proteins .

Link to Published Version: https://doi.org/10.1007/s12602-021-09771-x
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Abstract

The effects of different levels of dietary Enterococcus casseliflavus (EC-001), as a potential probiotic, were investigated on the growth performance, hemato-biochemical parameters, immune responses, and resistance to Aeromonas hydrophila infection in common carp (Cyprinus carpio) fingerlings. Accordingly, fish (N = 720; 12.0 ± 0.5 g) were distributed into four treatments receiving different dietary levels of E. casseliflavus, EC-001 (0 [control], 1 × 107, 108, and 109 CFU g−1 feed), for 8 weeks. The fish fed with a diet containing 109 CFU g−1 showed the highest weight gain and specific growth rate, along with the lowest feed conversion ratio, compared with the control group (P < 0.05). Red and white blood cells, hemoglobin, hematocrit, neutrophils, and monocytes significantly increased in the fish fed with 1 × 108 and 109 CFU g−1 (P < 0.05). Dietary inclusion of 1 × 108 and 109 CFU g−1 significantly increased serum total protein, albumin, and immunoglobulin content (P < 0.05). Feeding the fish with 1 × 109 CFU g−1 resulted in a significant increase in serum and skin mucus lysozyme activity compared with the other groups (P < 0.05). Complement component 3 and skin mucus protease activity were also significantly higher in all the fish treated with dietary E. casseliflavus (EC-001) compared with the control group (P < 0.05). The cumulative mortality in the treated fish was lower (ranging from 10 to 22%) than the control group (31%) after challenging the fish with A. hydrophila infection, while the fish fed with E. casseliflavus (EC-001) at 1 × 109 CFU g−1 exhibited the lowest mortality rate (P < 0.05). In conclusion, our results revealed the potential probiotic effects of E. casseliflavus (EC-001) for enhancing growth performance, immunity, and disease resistance of common carp.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Sustainable Aquatic Ecosystems
Freshwater Fish Group & Fish Health Unit
Harry Butler Institute
Publisher: Springer New York LLC
Copyright: © 2021 Springer Nature Switzerland AG.
URI: http://researchrepository.murdoch.edu.au/id/eprint/60249
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