Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Masculinization of Zebrafish through partial depletion of primordial germ by injecting diluted oligonucleotides into Embryos

Orbán, L., Saju, J.M., Tzung, K-W and Liew, W.C. (2021) Masculinization of Zebrafish through partial depletion of primordial germ by injecting diluted oligonucleotides into Embryos. In: Dosch, R., (ed.) Germline Development in the Zebrafish. Humana Press, pp. 49-60.

Link to Published Version: https://doi.org/10.1007/978-1-0716-0970-5_5
*Subscription may be required

Abstract

The regulation of reproduction in zebrafish, the prime model of fish research, is not fully understood. An efficient tool to gain a better understanding of this complicated process is utilization of severely sex-biased families or groups. Here, we describe a method for partial depletion of primordial germ cells (PGCs) that leads to eventual masculinization of zebrafish. The technique is based on injecting early embryos with diluted morpholino oligonucleotides that temporarily interfere with the production of Dead end (Dnd), an RNA-binding protein essential for PGC survival. In addition, we also propose the use of eviscerated trunk, as a suitable alternative for examining gonadal expression in juvenile zebrafish.

Item Type: Book Chapter
Murdoch Affiliation(s): Centre for Comparative Genomics
Publisher: Humana Press
Other Information: Part of the Methods in Molecular Biology book series (MIMB, volume 2218)
URI: http://researchrepository.murdoch.edu.au/id/eprint/60018
Item Control Page Item Control Page