Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Impact of expression mode and timing of sample collection, relative to milk ejection, on human milk bacterial DNA profiles

Cheema, A.S., Lai, C.T., Dymock, M., Rea, A., Geddes, D.T., Payne, M.S. and Stinson, L.F. (2021) Impact of expression mode and timing of sample collection, relative to milk ejection, on human milk bacterial DNA profiles. Journal of Applied Microbiology . Early View.

Link to Published Version: https://doi.org/10.1111/jam.14998
*Subscription may be required

Abstract

Aim
To investigate the impact of expression mode: electric breast pump or hand expression, and timing of sample collection: pre‐ and post‐milk ejection on human milk (HM) bacterial DNA profiles.

Methods and results
Three HM samples from the same breast were collected from 30 breastfeeding mothers: a pre‐milk ejection pump‐expressed sample (pre‐pump), a post‐milk ejection pump‐expressed sample (post‐pump) and a post‐milk ejection hand‐expressed sample (post‐hand). Full‐length 16S rRNA gene sequencing was used to assess milk bacterial DNA profiles. Bacterial profiles did not differ significantly based on mode of expression nor timing of sample collection. No significant differences were detected in the relative abundance of any OTUs based on expression condition (pre‐pump/ post‐pump and post‐pump/post‐hand) with univariate linear mixed‐effects regression analyses (all P‐values > 0·01; α = 0·01). Similarly, no difference in richness was observed between sample types (number of observed OTUs: post‐pump/post‐hand P = 0·13; pre‐pump/post‐pump P = 0. 45).

Conclusion
Bacterial DNA profiles of HM did not differ according to either expression method or timing of sample collection.

Significance and Impact of the Study
Hand or pump expression can be utilized to collect samples for microbiome studies. This has implications for the design of future HM microbiome studies.

Item Type: Journal Article
Murdoch Affiliation(s): Information Technology, Mathematics and Statistics
Publisher: Wiley
Copyright: © 2021 The Society for Applied Microbiology
URI: http://researchrepository.murdoch.edu.au/id/eprint/59513
Item Control Page Item Control Page