Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Enhanced nodulation and symbiotic effectiveness of Medicago truncatula when co-inoculated with Pseudomonas fluorescens WSM3457 and Ensifer (Sinorhizobium) medicae WSM419

Fox, S.L., O'Hara, G.W. and Bräu, L. (2011) Enhanced nodulation and symbiotic effectiveness of Medicago truncatula when co-inoculated with Pseudomonas fluorescens WSM3457 and Ensifer (Sinorhizobium) medicae WSM419. Plant and Soil, 348 (1-2). pp. 245-254.

[img]
Preview
PDF - Published Version
Download (295kB) | Preview
Free to read: http://dx.doi.org/10.1007/s11104-011-0959-8
*No subscription required

Abstract

Aims: Low numbers of rhizobia in soil or inoculants delay nodulation and decrease symbiotic legume productivity. This study investigated the effect of co-inoculation with a helper bacterium, Pseudomonas fluorescens WSM3457 on the Medicago truncatula - Ensifer (Sinorhizobium) medicae WSM419 symbiosis challenged by a low inoculum dose. Methods: In a glasshouse experiment the effect of co-inoculation with WSM3457 on the kinetics of nodule initiation and development was assessed 5,7,10,14,1721, and 42 days after inoculation of M. truncatula cv. Caliph with 103 cells/plant of E. medicae WSM419. Results: Co-inoculated plants had enhanced rate of nodule initiation and development, greater numbers of larger crown nodules, and by day 42 accumulated more N than plants inoculated with E. medicae WSM419 alone. Nodule development was altered by co-inoculation. Approximately 25% of nodule initials on co-inoculated plants formed in closely associated pairs, young nodules were larger with multiple meristems and developed into cluster-like multi-lobed nodules compared to those on WSM419 inoculated plants. Molecular typing showed WSM3457 occupied a significant proportion of root nodules on co-inoculated plants. Conclusion: Co-inoculation with P. fluorescens WSM3457 enhanced symbiotic effectiveness of M. truncatula when inoculated with a low inoculum dose of E. medicae WSM419.

Item Type: Journal Article
Murdoch Affiliation(s): Centre for Rhizobium Studies
School of Biological Sciences and Biotechnology
Publisher: Kluwer Academic Publishers
Copyright: © 2011 The Author(s).
URI: http://researchrepository.murdoch.edu.au/id/eprint/5834
Item Control Page Item Control Page

Downloads

Downloads per month over past year