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Cryptosporidium bollandi n. sp. (Apicomplexa: Cryptosporidiiae) from angelfish (Pterophyllum scalare) and Oscar fish (Astronotus ocellatus)

Bolland, S.J., Zahedi, A.ORCID: 0000-0002-0165-3797, Oskam, C., Murphy, B. and Ryan, U.ORCID: 0000-0003-2710-9324 (2020) Cryptosporidium bollandi n. sp. (Apicomplexa: Cryptosporidiiae) from angelfish (Pterophyllum scalare) and Oscar fish (Astronotus ocellatus). Experimental Parasitology, 217 . Art. 107956.

Link to Published Version: https://doi.org/10.1016/j.exppara.2020.107956
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Abstract

The species name Cryptosporidium bollandi n. sp. is proposed for Cryptosporidium piscine genotype 2 based on morphological, biological and molecular characterisation. Phylogenetic analyses of 18S rRNA (18S) sequences revealed that C. bollandi n. sp. was most closely related to piscine genotype 4 (5.1% genetic distance) and exhibited genetic distances of 10.0%, 12.2% and 25.2% from Cryptosporidium molnari, Cryptosporidium huwi and Cryptosporidium scophthtalmi, respectively. At the actin locus, C. bollandi n. sp. was again most closely related to piscine genotype 4 (6.8% genetic distance) and exhibited 15.5% (C. molnari), 18.4% (C. huwi), 22.9% (C. scophthalmi) and up to 27.5% genetic distance from other Cryptosporidium spp. (Cryptosporidium felis). Phylogenetic analysis of concatenated 18S and actin sequences showed that C. bollandi n. sp. exhibited 12.9% (C. molnari) to 21.1% (C. canis) genetic distance from all other Cryptosporidium spp. Genetic data as well as previous histological analysis clearly supports the validity of C. bollandi n. sp. as a separate species.

Item Type: Journal Article
Murdoch Affiliation: College of Science, Health, Engineering and Education
Vector and Waterborne Pathogens Research Group
Publisher: Academic Press
Copyright: © 2020 Elsevier Inc.
URI: http://researchrepository.murdoch.edu.au/id/eprint/56895
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