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Assessment of the pest status of Pratylenchus curvicauda and ultrastructural changes in roots of infected wheat and barley

Begum, F., Jones, M.G.K.ORCID: 0000-0001-5002-0227 and Fosu-Nyarko, J. (2020) Assessment of the pest status of Pratylenchus curvicauda and ultrastructural changes in roots of infected wheat and barley. Plant Pathology, 69 (8). pp. 1574-1588.

Link to Published Version: https://doi.org/10.1111/ppa.13232
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Abstract

Pratylenchus curvicauda , which was first described in metropolitan Perth in 1991, was recently identified in grain‐growing areas in Western Australia. The biology of this root‐lesion nematode, and especially its pest status, is unknown. We investigated its life cycle and interaction with host plants, because such information is essential for its management. The life cycle took 45 days to complete in a wheat cultivar maintained at 23°C. Over 10 weeks, the nematode multiplied in 26 of 61 genotypes; these host plants were all cereals and included widely grown cultivars of wheat and barley. Eighteen other cereal genotypes and 13 cultivars including canola, chickpea, ryegrass, lupin, soybean, and tomato, sustained the nematodes to different degrees without multiplication. Four cover crops were not suitable hosts. The patterns of attraction of the nematodes and penetration into roots of the host and tolerant plants were similar. The nonhosts attracted fewer nematodes, none of which penetrated the roots. Browning of infected roots was atypical—it occurred late in some roots, 55 days after inoculation, and in the presence of a fungus. The nematodes were confined to, and fed from, cortical cells. The ultrastructure of infected wheat and barley cells showed typical signs of damage caused by Pratylenchus spp. and included cell disorganization and lack of membrane integrity, disintegration of cytoplasm, hypertrophy of some nuclei, and deposition of tannin‐like granules. This detailed characterization of P . curvicauda– host interaction indicates the nematode is likely to be a pest of major crops, and attention should be given to its management.

Item Type: Journal Article
Murdoch Affiliation: College of Science, Health, Engineering and Education
Western Australian State Agricultural Biotechnology Centre
Publisher: Wiley-Blackwell
Copyright: © 2020 British Society for Plant Pathology
URI: http://researchrepository.murdoch.edu.au/id/eprint/56795
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