Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Definition of peptide binding motifs amongst the HLA-A*30 allelic group

Krausa, P., Munz, C., Keilholz, W., Stevanovic, S., Jones, E.Y., Browning, M., Bunce, M., Rammensee, H-G and McMichael, A. (2000) Definition of peptide binding motifs amongst the HLA-A*30 allelic group. Tissue Antigens, 56 (1). pp. 10-18.

Link to Published Version:
*Subscription may be required


HLA class I molecules present endogenously processed peptide ligands for surveillance by the T-cell receptor. This potentially immunogenic surface of HLA and peptide is a consequence of the polymorphism found within the HLA molecule and its preference for ligand binding together with peptide conformation within the binding groove. To investigate the relation between the polymorphic differences between some closely related HLA alleles and their effect on peptide preference, transfectants were established, each containing one of four allelic variants of HLA-A*30. Peptides from all four transfectants were eluted, and both individual ligands and peptide pools were sequenced. The data shows two distinct peptide motifs which distinguish A*3001 from the other three known A*30 variants. Differences in preferences at minor positions within the peptide sequence were noted between A*3002, A*3003 and A*3004, providing additional evidence of the implications of sequence polymorphism to HLA function.

Item Type: Journal Article
Publisher: Blackwell Publishing
Copyright: 2000 Munksgaard
Item Control Page Item Control Page