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CD1 genotyping of patients with Mycobacterium Malmoense pulmonary disease

Jones, D.C., Gelder, C.M., Campbell, I.A, Barnardo, M.C.N.M., Marshall, S.E and Bunce, M. (2001) CD1 genotyping of patients with Mycobacterium Malmoense pulmonary disease. European Journal of Immunogenetics, 28 (2). p. 288.

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Mycobacterium malmoense is an opportunistic mycobacterium. Little is known about genetic susceptibility to opportunistic mycobacterial infection. As CD1 molecules are capable of presenting antigens from the more virulent M. tuberculosis to T-cells, we were interested to discover whether recently described polymorphisms in CD1 molecules modulated susceptibility to M. malmoense pulmonary disease. The CD1 system comprises of five genes (CD1A, B, C, D, and E) located on chromosome 1 (1q22-23). CD1 molecules are structurally and functionally related to class I molecules and are expressed on dedicated antigen presenting cells. The primary function of CD1 molecules is to present lipid and glycolipid antigens to T cells. We have developed an allele-specific PCR-SSP method of CD1 genotyping. Using this method, we compared the allele and haplotype frequencies of CD1 in 49 HIV negative patients with M. malmoense pulmonary disease with those in 176 M. malmoense negative normal control samples from local cadaver donors. The CD1A and CD1E alleles typed for were nominally identified as CD1A*01, CD1A*02, CD1E*01 and CD1E*02: the control gene frequencies were found to be 8.6%, 92.5%, 55.5% and 38.5% respectively. No significant difference was observed between the patient and control cohorts. Positive relative linkage disequilibrium values of 1.0 were observed between CD1A*01 and CD1E*02 (chi sq 19.24), CD1A*02 and CD1E*01 (chi sq 0.58) and CD1A*02 and CD1E*02 (chi sq .06).

Item Type: Journal Article
Publisher: John Wiley and Sons
Copyright: 2001 Blackwell Science Ltd.
Notes: 15th European Histocompatibility Conference Abstracts
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