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Mass spectrometry imaging (MSI) for plant metabolomics

Boughton, B.A.ORCID: 0000-0001-6342-9814 and Thinagaran, D. (2018) Mass spectrometry imaging (MSI) for plant metabolomics. In: António, C., (ed.) Plant Metabolomics. Humana Press, New York, NY, pp. 241-252.

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Mass spectrometry imaging (MSI) is a developing technique to measure the spatiotemporal distribution of many biomolecules in tissues. Over the preceding decade MSI has been adopted by plant biologists and applied in a broad range of areas including: primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids, and the developing field of spatial metabolomics. This methods chapter covers preparation of plant tissues for matrix-assisted laser desorption ionization (MALDI)-MSI, including sample embedding and freezing, sectioning, mounting, and matrix deposition using both sublimation and spray deposition prior to MSI analysis.

Item Type: Book Chapter
Publisher: Humana Press
Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2018
Other Information: Part of the Methods in Molecular Biology book series (MIMB, Volume 1778)
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