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The importance of sampling immature leaves for the diagnosis of boron deficiency in oilseed rape (Brassica napus cv. Eureka)

Huang, L., Ye, Z. and Bell, R.W.ORCID: 0000-0002-7756-3755 (1996) The importance of sampling immature leaves for the diagnosis of boron deficiency in oilseed rape (Brassica napus cv. Eureka). Plant and Soil, 183 (2). pp. 187-198.

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Plant analysis can diagnose boron (B) deficiency when the standards used have been properly developed by establishing that a close relationship exists between B concentration in a plant part and its physiological function. The purpose of the present study was to demonstrate the importance of choosing the growing immature leaves for B deficiency diagnosis and for establishing critical B concentrations for the diagnosis of B deficiency in oilseed rape (Brassica napus). In Experiment 1, the plants were subject to seven levels of B supply using programmed nutrient addition, for the estimation of critical B concentrations in plant parts for shoot growth. In Experiment 2, the plants were treated with two levels of B supply in solution: 10 (+B) and 0 (-B) uM B, for the estimation of functional B requirements for leaf elongation. The results showed that critical B concentrations varied amongst the plant parts sampled and decreased with leaf age. As B taken up by roots is largely phloem-immobile, B concentrations in mature leaves are physiologically irrelevant to plant B status at the time of sampling, giving rise to a significant over- or underestimation of the B requirement for plant growth. By contrast, a growing, immature leaf, in this case the youngest open leaf (YOL), was the most reliable plant part for B deficiency diagnosis. Critical B concentrations developed from both methods were comparable-i.e. 10–14 mg B kg–1 dry matter in the YOL at vegetative growth stages up to stem elongation.

Item Type: Journal Article
Murdoch Affiliation(s): School of Biological and Environmental Sciences
Publisher: Kluwer Academic Publishers
Copyright: 1996 Kluwer Academic Publishers
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