Analytical validation of a commercial Elisa for determination of canine procalcitonin

Rossi, G.ORCID: 0000-0003-4879-9504, Bertazzolo, W., Vitiello, T. and Paltrinieri, S. (2015) Analytical validation of a commercial Elisa for determination of canine procalcitonin. Veterinary Clinical Pathology, 44 (1). E18.

Abstract

Procalcitonin (ProCT) is a marker used in people to differentiate septic and nonseptic acute inflammation. The magnitude of ProCT concentration correlates with the severity of the disease and is useful as a prognostic and risk factor. A ProCT gene overexpression has been demonstrated in leukocytes of diseased dogs, thus it can be hypothesized that ProCT could be a useful marker of sepsis in dogs. Several commercial ELISA kits specific for canine ProCT are available, thus the aim of this study was to perform an analytical validation of an ELISA specific for canine ProCT. Dogs were selected as possibly septic based on C-reactive protein (CRP) concentration, hematology, biochemical data, and clinical history. Three pools with low, medium, and high CRP levels were measured 10 times each. Moreover, 22 samples selected based on results of the first plate were measured up to 5 times to assess intraassay precision. Seven samples were measured twice on 2 different plates to assess interassay repeatability. Accuracy was determined by linearity under dilution (LUD) and spiking/ recovery test. The results revealed extremely high coefficients of variation (CV) in both intra- and interassay precision (mean CV: 109.1 and 65.8, respectively). LUD correlated poorly between obtained and expected results (p = 0.12, r2 = 0.78), whereas spiking/recovery test revealed a good correlation (P < .0001, r2 = 0.99). In conclusion, this ELISA kit for determination of canine ProCT lacks precision and accuracy, especially at medium and low levels, and for these reasons its use cannot be recommended.

Item Type:	Journal Article
Publisher:	American Society for Veterinary Clinical Pathology
Copyright:	© 2015 American Society for Veterinary Clinical Pathology
Other Information:	Poster presentation give @ European Society of Veterinary Clinical Pathology (EXVCP/European College of Veterinary Clinical Pathology (ECVCP) 16th Annual Congress. Milan, Italy, October 1 - 4 2014
URI:	http://researchrepository.murdoch.edu.au/id/eprint/56110

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