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Preliminary results: Big Endothelin-1 in serum from dogs with kidney disease

Giordano, A., Rossi, G.ORCID: 0000-0003-4879-9504, Virtuoso, A., Zatelli, A. and Paltrinieri, S. (2009) Preliminary results: Big Endothelin-1 in serum from dogs with kidney disease. Veterinary Clinical Pathology, 38 (Supp. 1). E30.

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Background: Endothelin-1 (ET-1) is a potent vasoconstrictor potentially associated with microangiopathy and development of chronic kidney disease. It stimulates the rennin-angiotensinaldosterone system and has positive inotropic and chronotropic effects. ET-1 arises from its precursor bigET-1 which has a longer half-life and thus is more easily detectable in serum than ET-1. Objective: To determine the serum concentration of bigET-1 in dogs with chronic kidney disease (CKD) and to assess whether bigET-1 varies, depending on the presence of inflammation or on the severity of CKD. Methods: BigET-1 was measured using a commercially available ELISA kit on serum samples from 56 dogs staged according to the classification of the International Renal Interest Society (IRIS). C-reactive protein was measured to identify inflammation. Serology for Leishmania was also performed on 53 dogs. Results: The level of bigET-1 was higher (Po0.001) in dogs in stage IV (mean_SD.: 28.0_19.5; median: 21.8 pg/mL) compared with dogs in stage I (9.2_6.5; 7.9), II (8.2_3.4; 9.5) or III (10.4_6.1; 9.1) kidney disease. No significant differences were found among proteinuric dogs (19.2_18.1; 14.7), dogs with borderline proteinuria (13.4_9.3; 12.4) and nonproteinuric dogs (7.3_3.2; 6.9) or between dogs seropositive (15.8_25.6; 9.1) and seronegative (17.7_12.0; 15.6) for Leishmania. BigET-1 was correlated with creatinine (Po0.001; r2 = 0.68) and CRP (Po0.001; r2 = 0.52) but not with urinary protein/creatinine ratio (P = 0.051; r2 = 0.27). Conclusions: Serum bigET-1 increases in dogs with severe CKD of inflammatory origin. Leishmania infection does not affect bigET-1 concentration. The pathogenic mechanisms of increased bigET-1 and its possible association with clinically evident leishmaniasis should be further investigated.

Item Type: Journal Article
Publisher: American Society for Veterinary Clinical Pathology
Copyright: © 2009 American Society for Veterinary Clinical Pathology
Other Information: Oral platform presentations given @ European Society of Veterinary Clinical Pathology (ESVCP) and European College of Veterinary Clinical Pathology (ECVCP) 11th Annual Congress
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