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Evaluation of equine hemograms using ADVIA 120

Giordano, A., Rossi, G.ORCID: 0000-0003-4879-9504, Pieralisi, C. and Paltrinieri, S. (2006) Evaluation of equine hemograms using ADVIA 120. Veterinary Clinical Pathology, 35 (4). p. 474.

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The ADVIA 120 is an automated laser cell counter widely used in human medicine and in a variety of animal species. Although specific software for equine haematology is available and in the literature there are different reports about its use, only a few reports have been published about the comparison between the ADVIA 120 and other methods for equine hemogram evaluation. In the present work we compared the performance of the ADVIA 120 to those of an impedance cell counter (Hemat 8, SEAC) and to the microscopic evaluation of blood smears. We analysed blood samples from 114 standardbred healthy horses. Both techniques are frequently used in equine haematology. The analysis performed with the ADVIA 120 showed values higher than those of SEAC for RBC, WBC, Hb, RDW, MCH and MCHC and lower for Hct and platelets. These differences probably depend on the peculiar analytic technique of the ADVIA 120 system and, although significant, only minimal and poorly biologically important differences were detected. The greatest differences were in neutrophil and basophil differentials, which were higher with the ADVIA120 than microscopic analysis and in lymphocyte percentages which were lower with the ADVIA 120. The laser measurements should be considered more reliable because they are obtained on a larger number of cells. The agreement tests (Passing Bablock and Bland- Altman plot) showed a linear correlation for RBC and platelet number, while a constant and proportional bias was present for the other erythroid and leukocytes parameters. The main advantage consists in the possible ancillary information about HDW, CH, CHCM and reticulocyte and related parameter evaluation, usually not calculated in horses. Repeatability of the ADVIA 120 measurements was not always optimal: the CV related to erythroid parameters was always less than 3%, while platelets, leukocytes and reticulocytes CVs were often higher.

Item Type: Journal Article
Publisher: American Society for Veterinary Clinical Pathology
Other Information: Oral platform presentation given @ European Society of Veterinary Clinical Pathology (ESVCP) 8th Annual Congress, Cambridge, UK, 5 - 8 September 2006.
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