Murdoch University Research Repository

Welcome to the Murdoch University Research Repository

The Murdoch University Research Repository is an open access digital collection of research
created by Murdoch University staff, researchers and postgraduate students.

Learn more

Investigation on protective effect of recombinant protein (OmpTS) of Aeromonas hydrophila in Common carp ( Cyprinus carpio )

Eftekharmanavi, S., Peyghan, R., Soltani, M. and Ghorbanpoor Najafabadi, M. (2020) Investigation on protective effect of recombinant protein (OmpTS) of Aeromonas hydrophila in Common carp ( Cyprinus carpio ). Aquaculture Research . Early View.

Link to Published Version: https://doi.org/10.1111/are.14682
*Subscription may be required

Abstract

The outer membrane protein of Aeromonas hydrophila is a potential candidate for vaccine development. In this study, after cloning and expression of ompTS, 270 common carp, weighing 44 ± 5.7 g divided into five groups, were injected intraperitoneally twice with 3‐week intervals. Groups included the following: PBS, PBS plus Freund's adjuvant, recombinant protein, recombinant protein plus Freund's adjuvant and 20 fish as negative control. Two weeks after the second injection, 30 fish of each group were challenged with a dose of 2 × LD50 of Aeromonas hydrophila and RPS was measured. The antibody level was measured using ELISA test. The protection of recombinant protein in the immunized fish with and without adjuvant, respectively, was about 82.61% and 78.26% (the protection of recombinant protein electroeluted from an SDS–PAGE with and without adjuvant, respectively, was about 78.62% and 69.57%). The average of antibody level in recombinant protein with and without adjuvant was significantly higher than the PBS group (p < .05). The ability of recombinant ompTS to increase the antibody level and to protect the fish from challenge by A. hydrophila demonstrated that recombinant ompTS protein injection can be used to immunize common carp against A. hydrophila infection.

Item Type: Journal Article
Murdoch Affiliation: Centre for Sustainable Aquatic Ecosystems
Harry Butler Institute
Publisher: Blackwell Publishing Inc
Copyright: © 2020 John Wiley & Sons Ltd
URI: http://researchrepository.murdoch.edu.au/id/eprint/56014
Item Control Page Item Control Page